| 09.09.2020 16:30 | CS Unirea Ungheni 2018 | 4-1 | ACS Sticla Arieşul Turda | Central / Ungheni |
| 09.09.2020 16:30 | ACS Arieşul Mihai Viteazu | 0-3 | ACS Industria Galda | Arieşul / Mihai Viteazu |
| 09.09.2020 16:30 | CS Sănătatea Servicii Publice | 2-4 | FC Unirea Dej | Baza Sportivă Unirea (Jucu de mijloc) / Jucu |
| 09.09.2020 16:30 | CS Universitar din Alba Iulia | 3-5 | AFC Unirea 1924 | Mureşul / Vinţu de Jos |
| 09.09.2020 16:30 | CS Sportul 2007 Şimleu Silvaniei | 8-7 | SCM Zalău | Măgura / Şimleul Silvaniei |
| 09.09.2020 16:30 | ACS Poli Timişoara | 4-6 | CS Gloria Lunca-Teuz Cermei | Electrica / Timişoara |
| 09.09.2020 16:30 | ACS Pobeda Stár Bišnov | 4-2 | ACS Fortuna Becicherecu Mic | Hristo Stoichkov Arena / Dudeştii Vechi |
| 09.09.2020 16:30 | CSM Deva | 0-1 | CSC Ghiroda şi Giarmata Vii | CFR Simeria / Simeria |
| 09.09.2020 16:30 | CS Jiul Rovinari 2016 | 0-3 | ACSO Filiaşi | Tineretului / Rovinari |
| 09.09.2020 16:30 | ACS Minerul Costeşti | 0-5 | ACS Viitorul Dăeşti | 1 Mai - Municipal / Râmnicu Vâlcea |
| 09.09.2020 16:30 | SC Olimpia MCMXXI Satu Mare | 0-1 | CSM Sighetu Marmaţiei | Comunal / Dorolț-Dara |
| 09.09.2020 16:30 | CS Dante Botoşani | 0-3 | ACS Şomuz Fălticeni | Municipal / Dorohoi |
| 09.09.2020 16:30 | CSM Paşcani | 3-0 | FC Ozana Târgu Neamț | CFR / Paşcani |
| 09.09.2020 16:30 | AF CS Viitorul Curiţa | 0-3 | CSM Bacău | Măgura / Târgu Ocna |
| 09.09.2020 16:30 | CSL Victoria Gugeşti | 0-4 | CS Sporting Lieşti | Comunal / Gugeşti |
| 09.09.2020 16:30 | CSM Vaslui | 0-5 | ACS Huşana Huşi | Municipal / Vaslui |
| 09.09.2020 16:30 | CS Victoria Traian | 0-3 | CS Făurei | Mircea Ceicu / Traian |
| 09.09.2020 16:30 | ACS Flacăra Mihail Kogălniceanu | 0-4 | ACS Dacia Unirea Brăila | Mihail Kogălniceanu / Mihail Kogălniceanu |
| 09.09.2020 16:30 | CS Medgidia | 0-3 | CSA Axiopolis Sport Cernavodă | Iftimie Elisei / Medgidia |
| 09.09.2020 16:30 | CS Victoria Cumpăna | 3-0 | CS Poseidon Limanu-2 Mai | Centenar 2018 / Cumpăna |
| 09.09.2020 16:30 | CSM Râmnicu Sărat | 1-2 | AFC Metalul Buzău | Municipal / Râmnicu Sărat |
| 09.09.2020 16:30 | AS Înfrăţirea Jilavele | 1-3 | ACS Recolta Gheorghe Doja | Jilavele / Jilavele |
| 09.09.2020 16:30 | ACSM Olteniţa | 0-3 | AS FC Agricola Borcea | Orășenesc / Olteniţa |
| 09.09.2020 16:30 | Clubul Sportiv al Armatei Steaua | 6-0 | CS Baloteşti | Complex Sportiv Ghencea Teren 5 / Bucureşti |
| 09.09.2020 16:30 | SCM Dunărea 2020 Giurgiu | 1-7 | CS Sporting Roşiori 2008 | Dunărea Port / Giurgiu |
| 09.09.2020 16:30 | AS Gloria 2015 Corneşti | 0-3 | AS FC Pucioasa | Comunal Corneşti / Corneşti |
| 09.09.2020 16:30 | ASC Olimpic Zărneşti | 1-0 | ACS Olimpic Cetate Râşnov | Celuloza / Zărneşti |
| 09.09.2020 16:30 | ACS KSE Târgu Secuiesc | 4-6 | ACS SR Municipal Braşov | Doctor Sinkovits / Târgu Secuiesc |
| 09.09.2020 16:30 | AFC Odorheiu Secuiesc | 2-0 | CSM Avântul Reghin | Municipal iarbă / Odorheiu Secuiesc |