| 28.07.2021 17:30 | AF CS Viitorul Curiţa | 2-0 | CSM Bacău | Energia / Oneşti |
| 28.07.2021 17:30 | CS Moldova Cristești | 1-4 | CS Dante Botoşani | Comunal / Cristești |
| 28.07.2021 17:30 | CSM Paşcani | 1-3 | ACS Huşana Huşi | CFR / Paşcani |
| 28.07.2021 17:30 | ACS Juniorul Suceava | 2-3 | ACS Şomuz Fălticeni | 1 Mai / Iţcani |
| 28.07.2021 17:30 | CSM Vaslui | 0-3 | CS Sporting Juniorul Vaslui | Municipal / Vaslui |
| 28.07.2021 17:30 | CS Victoria Traian | 2-4 | CS Viitorul Ianca | Mircea Ceicu / Traian |
| 28.07.2021 17:30 | AS Înfrăţirea Jilavele | 0-3 | ACS Recolta Gheorghe Doja | Jilavele / Jilavele |
| 28.07.2021 17:30 | ACS Pescăruşul Sarichioi Junior | 3-0 | ACS Sportul Chiscani | Pescăruş Arena / Sarichioi |
| 28.07.2021 17:30 | CS Năvodari | 4-0 | CS Gloria Albeşti | Flacăra / Năvodari |
| 28.07.2021 17:30 | AS Zimbrul Slobozia Conachi | 1-6 | CS Sporting Lieşti | Dunărea 2 / Galaţi |
| 28.07.2021 17:30 | AS Phoenix Poşta Câlnău | 1-3 | CSO Plopeni | Ştiinţa / Aldeni |
| 28.07.2021 17:30 | ACS Inizio Focşani | 4-2 | ACS KSE Târgu Secuiesc | Comunal / Gugeşti |
| 28.07.2021 17:30 | AFC Dunărea Ciocăneşti | 6-5 | ACS Înainte Modelu | Dunărea / Ciocăneşti |
| 28.07.2021 17:30 | CS Făurei | 5-6 | AS FC Agricola Borcea | Orăşenesc / Făurei |
| 28.07.2021 17:30 | CS Brăneşti | 0-2 | CS Tunari | Cătălin Hâldan / Brăneşti |
| 28.07.2021 17:30 | ASCS Petrolul 95 | 4-6 | CSM Flacăra Moreni | Conpet / Strejnicu |
| 28.07.2021 17:30 | ACS Maxima Hobaia | 0-3 | CSM Alexandria | Comunal / Mihai Vodă |
| 28.07.2021 17:30 | CS Cârcea | 1-0 | CSO Petrolul Potcoava | Comunal Cârcea 1 / Cârcea |
| 28.07.2021 17:30 | ACS Viitorul Şimian | 1-3 | CS Gilortul Târgu Cărbuneşti | Municipal / Drobeta-Turnu Severin |
| 28.07.2021 17:30 | ATCS Sparta Râmnicu Vâlcea | 3-0 | ACS Flacăra Horezu | ISport Arena (ex-Brothers Sport Hub) / Râmnicu Vâlcea |
| 28.07.2021 17:30 | ACS Minerul Costeşti | 1-3 | CSM Jiul Petroşani | Gheorghe Ciucioi / Fedeleşoiu |
| 28.07.2021 17:30 | CS Armata Aurul Brad | 4-5 | CS Hunedoara | Aurul / Brad |
| 28.07.2021 17:30 | ACS Poli Timişoara | 3-6 | CSM Deva | Electrica / Timişoara |
| 28.07.2021 17:30 | ACS Nera Bozovici | 1-2 | ACS Progresul Ezeriş | Nera / Bozovici |
| 28.07.2021 17:30 | CS Universitatea de Vest din Timişoara | 1-2 | CSC Ghiroda şi Giarmata Vii | Ciarda Roşie / Timişoara |
| 28.07.2021 17:30 | CS Gloria Lunca-Teuz Cermei | 1-3 | CS Lotus Băile Felix | Comunal-Central / Cermei |
| 28.07.2021 17:30 | CS Foresta Tileagd | 2-1 | CS Luceafărul Oradea | Foresta Tileagd / Tileagd |
| 28.07.2021 17:30 | ACS Sportul Beclean | 3-0 | ACS FC Someşul Dej | Orășenesc / Beclean |
| 28.07.2021 17:30 | ACS Floreşti | 2-5 | CS Sănătatea Servicii Publice | Unirea / Floreşti |
| 28.07.2021 17:30 | ASCF Lăpuş R | 0-2 | CS Progresul Şomcuta Mare | Lăpuşul / Târgu Lăpuş |
| 28.07.2021 17:30 | AFC Ştiinţa Beltiug | 0-5 | CSM Satu Mare | Comunal / Beltiug |
| 28.07.2021 17:30 | CS Rapid Jibou | 0-5 | CS Sportul 2007 Şimleu Silvaniei | Rapid / Jibou |
| 28.07.2021 17:30 | AS Voinţa Stremţ | 2-0 | CS Ocna Mureş | Comunal / Stremţ |
| 28.07.2021 17:30 | ACS Industria Galda | 2-0 | AFC Unirea 1924 | Galda Arena / Galda de Jos |
| 28.07.2021 17:30 | ACS Steagul Roşu Braşov | 0-2 | ACS Kids Tâmpa 2015 Braşov | Unirea / Tărlungeni |
| 28.07.2021 17:30 | ACS FC Avrig | 4-1 | ACS Olimpic Cetate Râşnov | Baza sportivă a Oraşului Avrig / Avrig |
| 28.07.2021 17:30 | ACS Kinder Sângeorgiu de Mureş | 0-3 | CS Unirea Ungheni 2018 | Comunal / Gorneşti |
| 28.07.2021 17:30 | AS Golimpiakosz Odorheiu Secuiesc | 0-6 | AFC Odorheiu Secuiesc | Municipal sintetic / Odorheiu Secuiesc |
| 28.07.2021 17:30 | AS Inter Aninoasa | 2-7 | AS FC Pucioasa | Şcoala Broşteni / Aninoasa |
| 28.07.2021 17:30 | AFC Asalt Bucureşti | 8-0 | ACSM Olteniţa | Electromagnetica / Bucureşti |