| 11.08.2021 17:30 | CS Dante Botoşani | 3-1 | ACS Foresta Suceava | Municipal / Dorohoi |
| 11.08.2021 17:30 | ACS Şomuz Fălticeni | 3-0 | CSM Bucovina Rădăuţi | Municipal Constantin Jamaischi / Fălticeni |
| 11.08.2021 17:30 | CS Ştiinţa Miroslava | 10-9 | ACSM Ceahlăul Piatra Neamţ | Uricani - terenul 2 / Uricani |
| 11.08.2021 17:30 | CS Sporting Juniorul Vaslui | 1-2 | ACS Huşana Huşi | Municipal / Vaslui |
| 11.08.2021 17:30 | CS Sporting Lieşti | 0-4 | ACS Suporter Club Oţelul Galaţi | Comunal / Lieşti |
| 11.08.2021 17:30 | AF CS Viitorul Curiţa | 0-4 | AFC Odorheiu Secuiesc | Energia / Oneşti |
| 11.08.2021 17:30 | ACS Inizio Focşani | 0-2 | CSM Focşani 2007 | Comunal / Gugeşti |
| 11.08.2021 17:30 | ACS Recolta Gheorghe Doja | 1-0 | CS Viitorul Ianca | Comunal / Gheorghe Doja |
| 11.08.2021 17:30 | AFC Metalul Buzău | 2-0 | CSM Râmnicu Sărat | Metalul / Buzău |
| 11.08.2021 17:30 | CS Năvodari | 3-0 | CSA Axiopolis Sport Cernavodă | Flacăra / Năvodari |
| 11.08.2021 17:30 | AFC Dunărea Ciocăneşti | 3-5 | AS FC Agricola Borcea | Dunărea / Ciocăneşti |
| 11.08.2021 17:30 | SC Popeşti-Leordeni | 4-3 | CS Afumaţi | Inter Gaz / Popeşti-Leordeni |
| 11.08.2021 17:30 | AFC Asalt Bucureşti | 1-7 | CS Tunari | Electromagnetica / Bucureşti |
| 11.08.2021 17:30 | CSM Alexandria | 1-3 | CS Sporting Roşiori 2008 | CSS Peco / Alexandria |
| 11.08.2021 17:30 | CSO Plopeni | 4-1 | CS Blejoi | Gheorghe Şilaev / Plopeni |
| 11.08.2021 17:30 | CSM Flacăra Moreni | 0-2 | AS FC Pucioasa | Flacăra / Moreni |
| 11.08.2021 17:30 | ATCS Sparta Râmnicu Vâlcea | 1-2 | ACS Unirea Bascov | ISport Arena (ex-Brothers Sport Hub) / Râmnicu Vâlcea |
| 11.08.2021 17:30 | ACS Viitorul Dăeşti | 0-1 | ACS Vediţa Coloneşti M.S. | Gheorghe Ciucioi / Fedeleşoiu |
| 11.08.2021 17:30 | CS Cârcea | 0-3 | ACSO Filiaşi | Comunal Cârcea 1 / Cârcea |
| 11.08.2021 17:30 | ACS FC Avrig | 2-7 | Fotbal Club Braşov | Baza sportivă a Oraşului Avrig / Avrig |
| 11.08.2021 17:30 | ACS Kids Tâmpa 2015 Braşov | 7-8 | ACS SR Municipal Braşov | ICIM iarbă / Braşov |
| 11.08.2021 17:30 | CSM Jiul Petroşani | 6-1 | CS Gilortul Târgu Cărbuneşti | Petre Libardi / Petroşani |
| 11.08.2021 17:30 | CS Hunedoara | 5-3 | CSM Deva | Complex Sportiv "Corvinul 1921 Hunedoara" / Hunedoara |
| 11.08.2021 17:30 | ACS Progresul Ezeriş | 2-1 | CSC Ghiroda şi Giarmata Vii | Municipal / Caransebeş |
| 11.08.2021 17:30 | CSC Dumbrăviţa | 0-1 | CS Şoimii Lipova | Ştefan Dobay / Dumbrăviţa |
| 11.08.2021 17:30 | CS Avântul Periam | 2-1 | ACS Progresul Pecica | Comunal / Periam |
| 11.08.2021 17:30 | CS Lotus Băile Felix | 0-1 | CS Crişul Chişineu Criş | Lotus / Sânmartin |
| 11.08.2021 17:30 | CS Foresta Tileagd | 0-5 | Asociaţia Club Sportiv CAO 1910 | Foresta Tileagd / Tileagd |
| 11.08.2021 17:30 | CSM Satu Mare | 0-2 | CS Minaur Baia Mare | Olimpia - Daniel Prodan / Satu Mare |
| 11.08.2021 17:30 | CS Progresul Şomcuta Mare | 2-3 | CS Gloria 2018 Bistriţa-Năsăud | Recea Central / Recea |
| 11.08.2021 17:30 | CS Sportul 2007 Şimleu Silvaniei | 0-1 | SCM Zalău | Măgura / Şimleul Silvaniei |
| 11.08.2021 17:30 | ACS Sportul Beclean | 1-3 | FC Unirea Dej | Orășenesc / Beclean |
| 11.08.2021 17:30 | CS Sănătatea Servicii Publice | 3-2 | ACS Sticla Arieşul Turda | Baza Sportivă Unirea (Jucu de mijloc) / Jucu |
| 11.08.2021 17:30 | AS Voinţa Stremţ | 1-0 | ACS Industria Galda | Comunal / Stremţ |
| 11.08.2021 17:30 | CS Metalurgistul Cugir | 1-2 | CSC 1599 Şelimbăr | Arena CSO / Cugir |
| 11.08.2021 17:30 | CS Unirea Ungheni 2018 | 4-2 | CSM Avântul Reghin | Central / Ungheni |
| 18.08.2021 17:00 | AFC Progresul 1944 Spartac | 2-1 | Clubul Sportiv al Armatei Steaua | Progresul Spartac / Bucureşti |
| 18.08.2021 17:30 | ACS Pescăruşul Sarichioi Junior | 0-2 | AFC 1919 Dacia Unirea Brăila | Pescăruş Arena / Sarichioi |