| 24.08.2021 17:30 | CS Crişul Chişineu Criş | 0-2 | CS Şoimii Lipova | Crişul / Chişineu Criş |
| 24.08.2021 17:30 | AFC Odorheiu Secuiesc | 3-1 | CS Unirea Ungheni 2018 | Municipal iarbă / Odorheiu Secuiesc |
| 24.08.2021 17:30 | CS Gloria 2018 Bistriţa-Năsăud | 2-4 | FC Unirea Dej | Complexul Sportiv Jean Pădureanu / Bistriţa |
| 24.08.2021 17:30 | CSM Focşani 2007 | 4-6 | CS Aerostar Bacău | Milcovul / Focşani |
| 24.08.2021 17:30 | AFC Metalul Buzău | 1-3 | AS Fotbal Club Buzău | Metalul / Buzău |
| 24.08.2021 17:30 | ACS Recolta Gheorghe Doja | 3-1 | AS FC Agricola Borcea | Comunal / Gheorghe Doja |
| 24.08.2021 17:30 | CS Tunari | 1-0 | FC Metaloglobus Bucureşti | Concordia / Chiajna |
| 24.08.2021 17:30 | ACS SR Municipal Braşov | 1-0 | Fotbal Club Braşov | Tineretului / Braşov |
| 24.08.2021 17:30 | AS FC Pucioasa | 0-2 | ACS Unirea Bascov | Dumitru Tică Popescu / Pucioasa |
| 24.08.2021 17:30 | CS Sporting Roşiori 2008 | 0-1 | CSM Slatina | Municipal / Roşiori de Vede |
| 24.08.2021 17:30 | ACSO Filiaşi | 11-10 | ACS Vediţa Coloneşti M.S. | Orăşenesc / Filiaşi |
| 24.08.2021 17:30 | CSM Jiul Petroşani | 0-5 | CS Hunedoara | Petre Libardi / Petroşani |
| 25.08.2021 17:30 | CS Sănătatea Servicii Publice | 0-4 | AS FC Universitatea Cluj | Baza Sportivă Unirea (Jucu de mijloc) / Jucu |
| 25.08.2021 17:30 | Asociaţia Club Sportiv CAO 1910 | 1-0 | SCM Zalău | Iuliu Bodola / Oradea |
| 25.08.2021 17:30 | ACS Fotbal Comuna Recea | 0-3 | CS Minaur Baia Mare | - |
| 25.08.2021 17:30 | ACS Şomuz Fălticeni | 3-1 | CS Dante Botoşani | Municipal Constantin Jamaischi / Fălticeni |
| 25.08.2021 17:30 | ACS Huşana Huşi | 2-1 | CS Ştiinţa Miroslava | Municipal / Huşi |
| 25.08.2021 17:30 | AFC 1919 Dacia Unirea Brăila | 1-0 | ACS Suporter Club Oţelul Galaţi | Municipal / Brăila |
| 25.08.2021 17:30 | CS Năvodari | 3-1 | AFC Unirea Constanţa | Flacăra / Năvodari |
| 25.08.2021 17:30 | SC Popeşti-Leordeni | 0-1 | AFC Unirea 04 Slobozia | Inter Gaz / Popeşti-Leordeni |
| 25.08.2021 17:30 | AS Voinţa Stremţ | 0-4 | CSC 1599 Şelimbăr | Comunal / Stremţ |
| 25.08.2021 17:30 | ACS Progresul Ezeriş | 2-3 | Clubul Sportiv Muncitoresc Reşiţa | Municipal / Caransebeş |
| 25.08.2021 17:30 | CS Avântul Periam | 1-4 | SC Fotbal Club Ripensia Timișoara | Comunal / Periam |
| 25.08.2021 20:00 | CS Pandurii Lignitul Târgu Jiu | 0-1 | ACS Viitorul Pandurii Târgu Jiu | Municipal Constantina Diţă / Târgu Jiu |
| 26.08.2021 17:30 | AFC Progresul 1944 Spartac | 5-6 | CS Concordia Chiajna | Progresul Spartac / Bucureşti |
| 01.09.2021 16:30 | CSO Plopeni | 0-5 | ACS Petrolul 52 | Gheorghe Şilaev / Plopeni |