| 17.08.2022 17:30 | ACS Sticla Arieşul Turda | 0-9 | ACS Academia de Fotbal Viitorul Cluj | Municipal / Turda |
| 17.08.2022 17:30 | CS Sănătatea Servicii Publice | 3-4 | CS Minerul 1947 Ocna Dej | Clujana / Cluj-Napoca |
| 17.08.2022 17:30 | CS Sportul 2007 Şimleu Silvaniei | 4-2 | SCM Zalău | Măgura / Şimleul Silvaniei |
| 17.08.2022 17:30 | CS Progresul Şomcuta Mare | 1-12 | CSM Satu Mare | Progresul / Şomcuta Mare |
| 17.08.2022 17:30 | CSM Avântul Reghin | 1-2 | CS Gloria 2018 Bistriţa-Năsăud | Avântul / Reghin |
| 17.08.2022 17:30 | ACS Tg. Mureş 1898 - MSE 1898 | 1-5 | CS Unirea Ungheni 2018 | Academia de Sport Mureşul (Complex Weekend) / Târgu Mureş |
| 17.08.2022 17:30 | CSO Viitorul Darabani | 5-4 | CSM Bucovina Rădăuţi | Stadionul Orăşenesc Chirica Puşcaşu / Darabani |
| 17.08.2022 17:30 | ACS Şomuz Fălticeni | 2-1 | ACSM Ceahlăul Piatra Neamţ | Municipal Constantin Jamaischi / Fălticeni |
| 17.08.2022 17:30 | CSM Paşcani | 1-2 | CS Ştiinţa Miroslava | CFR / Paşcani |
| 17.08.2022 17:30 | CSM Bacău | 3-4 | AFC Dinamo Bacău | Constantin Anghelache / Bacău |
| 17.08.2022 17:30 | CS Sporting Lieşti | 0-2 | CS Aerostar Bacău | Comunal / Lieşti |
| 17.08.2022 17:30 | CSM Râmnicu Sărat | 2-3 | AFC Metalul Buzău | Municipal / Râmnicu Sărat |
| 17.08.2022 17:30 | ACS Sportul Chiscani | 1-5 | CS Viitorul Ianca | Sportul / Chiscani |
| 17.08.2022 17:30 | CS Făurei | 2-5 | ACS Recolta Gheorghe Doja | Orăşenesc / Făurei |
| 17.08.2022 17:30 | CSM Feteşti | 0-1 | AS FC Agricola Borcea | Tineretului / Feteşti |
| 17.08.2022 17:30 | ACSM Olteniţa | 0-1 | ACS Înainte Modelu | Orășenesc / Olteniţa |
| 17.08.2022 17:30 | CSM Cetatea Turnu Măgurele | 1-2 | CSM Alexandria | Municipal / Turnu Măgurele |
| 17.08.2022 17:30 | CSO Petrolul Potcoava | 4-3 | CS Sporting Roşiori 2008 | Petrolul / Potcoava |
| 17.08.2022 17:30 | ACSO Filiaşi | 3-1 | ACS Vediţa Coloneşti | Orăşenesc / Filiaşi |
| 17.08.2022 17:30 | CS Real Bradu | 0-1 | ACS Unirea Bascov | Central Bradu / Bradu |
| 17.08.2022 17:30 | ACS Atletic United (Uniţi) Ploieşti 1906 | 0-4 | CSM Flacăra Moreni | Flacăra / Moreni |
| 17.08.2022 17:30 | CSO Plopeni | 0-1 | CS Blejoi | Gheorghe Şilaev / Plopeni |
| 17.08.2022 17:30 | ACS Kids Tâmpa 2015 Braşov | 0-1 | ACS KSE Târgu Secuiesc | ICIM iarbă / Braşov |
| 17.08.2022 17:30 | ACS SR Municipal Braşov | 4-0 | ACS Olimpic Cetate Râşnov | Tineretului / Braşov |
| 17.08.2022 17:30 | ACS Minerul Costeşti | 0-2 | ACS Flacăra Horezu | ISport Arena (ex-Brothers Sport Hub) / Râmnicu Vâlcea |
| 17.08.2022 17:30 | CS Gilortul Târgu Cărbuneşti | 3-0 | ACS Viitorul Şimian | Cristinel Răducan / Târgu Cărbuneşti |
| 17.08.2022 17:30 | CSM Jiul Petroşani | 7-1 | CS Pandurii Lignitul Târgu Jiu | Petre Libardi / Petroşani |
| 17.08.2022 17:30 | ACS Progresul Ezeriş | 1-3 | AFC Voinţa Lupac | Gloria / Reşiţa |
| 17.08.2022 17:30 | CSM Lugoj | 7-2 | CS Armata Aurul Brad | Municipal / Lugoj |
| 17.08.2022 17:30 | CS Avântul Periam | 3-2 | ACS Pobeda Stár Bišnov | Comunal / Periam |
| 17.08.2022 17:30 | CS Frontiera 2004 Curtici | 0-3 | ACS Progresul Pecica | Frontiera / Curtici |
| 17.08.2022 17:30 | CS Gloria Lunca-Teuz Cermei | 1-2 | CS Şoimii Lipova | Comunal-Central / Cermei |
| 17.08.2022 17:30 | CS Luceafărul Oradea | 2-9 | CS Crişul Chişineu Criş | Luceafărul / Sânmartin |
| 17.08.2022 17:30 | ACSC Crişul Sântandrei | 1-2 | CS Lotus Băile Felix | Crişul / Sântandrei |
| 17.08.2022 20:00 | CS Axiopolis Cernavodă | 2-1 | CS Gloria Albeşti | Ideal / Cernavodă |
| 20.08.2022 17:30 | AFC Unirea 1924 | 1-3 | CS Ocna Mureş | Cetate / Alba Iulia |